Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives from Toad and Cone Snail - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology Année : 2014

Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives from Toad and Cone Snail

Résumé

A nano-flow high-resolution screening platform, featuring a parallel chip-based microfluidic bioassay and mass spectrometry coupled to nano-liquid chromatography, was applied to screen animal venoms for nicotinic acetylcholine receptor like (nAChR) affinity by using the acetylcholine binding protein, a mimic of the nAChR. The potential of this microfluidic platform is demonstrated by profiling the Conus textile venom proteome, consisting of over 1,000 peptides. Within one analysis (<90 min, 500 ng venom injected), ligands are detected and identified. To show applicability for non-peptides, small molecular ligands such as steroidal ligands were identified in skin secretions from two toad species (Bufo alvarius and Bufo marinus). Bioactives from the toad samples were subsequently isolated by MS-guided fractionation. The fractions analyzed by NMR and a radioligand binding assay with α7-nAChR confirmed the identity and bioactivity of several new ligands.
Fichier principal
Vignette du fichier
Biology-Heus-2014.pdf (543.17 Ko) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02306963 , version 1 (07-10-2019)

Identifiants

Citer

Ferry Heus, Reka A Otvos, Ruud L E G Aspers, Rene van Elk, Jenny I Halff, et al.. Miniaturized Bioaffinity Assessment Coupled to Mass Spectrometry for Guided Purification of Bioactives from Toad and Cone Snail. Biology, 2014, 3 (1), pp.139-156. ⟨10.3390/biology3010139⟩. ⟨hal-02306963⟩
135 Consultations
44 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More