Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining - Archive ouverte HAL Access content directly
Journal Articles Biomedical optics express Year : 2019

Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining

Julien Wojak
Herve Rigneault

Abstract

We present for the first time one-to-one correspondence between standard hema-toxylin/eosin (H&E) stained tissue sections and stimulated Raman histology (SRH)-a label-free technique in which stimulated Raman scattering (SRS) and second harmonic generation (SHG) are combined to generate virtual H&E images. Experiments were performed on both human thin cryogenic slides from the gastrointestinal tract (GI) and thick freshly excised biopsies from endoscopic surgery. Results on cryogenic slides evidenced an excellent agreement between SRH and H&E images while the ones on biopsies established the relevance of SRH for rapid intraoperative histology to assist in surgical decision making.
Fichier principal
Vignette du fichier
boe-10-10-5378.pdf (15.1 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-02302729 , version 1 (19-12-2019)

Identifiers

Cite

Barbara Sarri, Flora Poizat, Sandro Heuke, Julien Wojak, Florence Franchi, et al.. Stimulated Raman histology: one to one comparison with standard hematoxylin and eosin staining. Biomedical optics express, 2019, 10 (10), pp.5378. ⟨10.1364/BOE.10.005378⟩. ⟨hal-02302729⟩
98 View
34 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More