The purpose of the study was to evaluate the factor structure and the reliability of the Prosocial Behavior Scale (PBS). To our knowledge, no factorial validity of the multifactorial structure of PBS has been published to date. The psychometric characteristics of the PBS were examined in several samples of French adolescents (aged 11–19, N1 = 1141, N2 = 1071, and N3 = 1640) using Confirmatory factor analyses (CFA). The original four-factor structure was not confirmed due to lack of discriminant validity. CFA led us to retain a two-factor solution with a good fit and a satisfactory reliability. Furthermore, the results support the convergent validity of the PBS: helping and caring dimensions were positively correlated with empathy. In addition, partial measurement invariance across gender and grade was attested. In conclusion, the results indicate that the French version of the PBS is a useful instrument for the assessment of prosocial behaviors in adolescence.