Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein - Archive ouverte HAL
Article Dans Une Revue Journal of visualized experiments : JoVE Année : 2019

Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein

Résumé

We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.
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Dates et versions

hal-02282630 , version 1 (17-06-2020)

Identifiants

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Béatrice Vallée, Michel Doudeau, Fabienne Godin, Hélène Bénédetti. Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein. Journal of visualized experiments : JoVE, 2019, 148, pp.e59820. ⟨10.3791/59820⟩. ⟨hal-02282630⟩
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