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Communication Dans Un Congrès Année : 2019

Impact of chemical ageing on the toxicity of biogenic and anthropogenic organic aerosols using model cells

Résumé

A large subset of PM2.5 is identified as organic matter (OM) and ranges from 20 to 60 % of the fine aerosol mass. The OM originates from both primary (directly emitted) and secondary sources (formed from gas-to-particle conversion). This last fraction, also called secondary organic aerosol (SOA) represents the main component of OM as it could account for 20 to 80 % of its mass. While representing subset of atmospheric particle load, very little is known about the health effect of SOA. The aim of this work was to study the impact of aerosol ageing on cell viability. Both, biogenic (from limonene) and anthropogenic (from naphthalene) SOA were generated with different oxidation ageing (related to different atmospheric residence time) using a potential aerosol mass (PAM) chamber, to simulate different aerosol age. SOA were collected, extracted and model cells (mouse fibroplast cells, 3T3-L1) were exposed to the extracts. Cell proliferation was measured over time in order to evaluate their viability. The results shown that both aged and fresh SOA inhibit cell proliferation (after media washout) and this inhibition was increased as a function of SOA ageing. These results were associated with chemical characterization, using high-resolution mass spectrometry in order to link chemical composition and cell mortality. This work shows that SOA can influence cell viability and that ageing is an important factor to take into consideration when evaluating the effect of aerosol on health. This work will be applied to a more realistic and complex biological system (e.g., lung cells).
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Dates et versions

hal-02276471 , version 1 (02-09-2019)

Identifiants

  • HAL Id : hal-02276471 , version 1

Citer

S. Tomaz, A. Geloen, D. Li, Y. Clémen, P. Lantéri, et al.. Impact of chemical ageing on the toxicity of biogenic and anthropogenic organic aerosols using model cells. European Aerosol Conference-EAC 2019, Aug 2019, Göteborg, Sweden. ⟨hal-02276471⟩
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