Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Nature Methods Année : 2019

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Résumé

Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort.

Dates et versions

hal-02270788 , version 1 (08-02-2024)

Identifiants

Citer

Elke de Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, et al.. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM. Nature Methods, 2019, 16 (8), pp.707-710. ⟨10.1038/s41592-019-0462-3⟩. ⟨hal-02270788⟩
78 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More