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            <funder>Te study was fnancially supported by the Ministry of Education, Youth and Sports of the Czech Republic projects CENAKVA (LM2018099) and Biodiversity CZ.02.1.01/0.0/0.0/16_025/0007370) and the Czech Science Foundation (grant number 17-19714Y)</funder>
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            <idno type="halRefHtml">&lt;i&gt;Scientific Reports&lt;/i&gt;, 2019, 9 (10453), pp.1-11. &lt;a target="_blank" href="https://dx.doi.org/10.1038/s41598-019-46892-4"&gt;&amp;#x27E8;10.1038/s41598-019-46892-4&amp;#x27E9;&lt;/a&gt;</idno>
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                <term xml:lang="en">fish</term>
                <term xml:lang="en">foetal development</term>
                <term xml:lang="en">cell cloning</term>
                <term xml:lang="en">nuclear transfer</term>
                <term xml:lang="en">somatic cell</term>
                <term xml:lang="en">species threatened with extinction</term>
                <term xml:lang="fr">reproduction</term>
                <term xml:lang="fr">histologie</term>
                <term xml:lang="fr">méthode de clonage</term>
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                <term xml:lang="fr">acipenseridae</term>
                <term xml:lang="fr">esturgeon commun</term>
                <term xml:lang="fr">clonage cellulaire</term>
                <term xml:lang="fr">conservation des espèces</term>
                <term xml:lang="fr">reprogrammation</term>
                <term xml:lang="fr">transfert nucléaire</term>
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              <p>Several steps of sturgeon somatic cell nuclear transfer (SCNT) have been recently established, but improvements are needed to make it a feasible tool to preserve the natural populations of this group of endangered species. The donor cell position inside the recipient egg seems to be crucial for its reprogramming; therefore by injecting multiple donor somatic cells instead of a single cell with a single manipulation, we increased the potential for embryo development. Using the Russian sturgeon Acipenser gueldenstaedtii as a multiple cell donor and sterlet Acipenser ruthenus as the non-enucleated egg recipient, we obtained higher proportion of eggs developing into embryos than previously reported with single-SCNT. Molecular data showed the production of a specimen (0.8%) contained only the donor genome with no contribution from the recipient, while two specimens (1.6%) showed both recipient and donor genome. These findings are the first report of donor DNA integration into a sturgeon embryo after interspecific cloning. In all, we provide evidence that cloning with the multiple donor somatic cells can be feasible in the future. Despite the fact that the sturgeon cloning faces limitations, to date it is the most promising technique for their preservation.</p>
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