Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol–Ene or Thiol–Yne Coupling Chemistry - Archive ouverte HAL
Article Dans Une Revue Bioconjugate Chemistry Année : 2016

Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol–Ene or Thiol–Yne Coupling Chemistry

Résumé

Nucleic acid microarray-based assay technology has shown lacks in reproducibility, reliability, and analytical sensitivity. Here, a new strategy of probe attachment modes for silicon-based materials is built up. Thus, hybridization ability is enhanced by combining thiol-ene or thiol-yne click chemistry reactions with a multipoint attachment of polythiolated probes. The viability and performance of this approach was demonstrated by specifically determining Salmonella PCR products up to a 20 pM sensitivity level.
Fichier principal
Vignette du fichier
Banuls%20et%20al%20Bioconj%20Chem_DEF.pdf (672.17 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02197214 , version 1 (31-01-2024)

Identifiants

Citer

Maria-Jose Bañuls, Pilar Jiménez-Meneses, Albert Meyer, Jean-Jacques Vasseur, François Morvan, et al.. Improved Performance of DNA Microarray Multiplex Hybridization Using Probes Anchored at Several Points by Thiol–Ene or Thiol–Yne Coupling Chemistry. Bioconjugate Chemistry, 2016, 28 (2), pp.496-506. ⟨10.1021/acs.bioconjchem.6b00624⟩. ⟨hal-02197214⟩
68 Consultations
34 Téléchargements

Altmetric

Partager

More