Extraction and Quantification of Lipids from Plant or Algae - Archive ouverte HAL
Chapitre D'ouvrage Année : 2018

Extraction and Quantification of Lipids from Plant or Algae

Valérie Gros
Josselin Lupette
Juliette Jouhet

Résumé

In plants and algae, the glycerolipidome changes in response to environmental modifications. For instance, in phosphate starvation, phospholipids are degraded and replaced by nonphosphorus lipids and in nitrogen starvation, storage lipids accumulate. In addition to the well-known applications of oil crops for food, algae lipids are becoming a model for potential applications in health, biofuel, and green chemistry and are used as a platform for genetic engineering. It is therefore important to measure accurately and quickly the glycerolipid content in plants and algae. Here we describe the methods to extract the lipid, quantify the fatty acid amount of the lipid extract and to quantify the different lipid classes that are present in these samples.
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Dates et versions

hal-02190907 , version 1 (23-07-2019)

Identifiants

Citer

Valérie Gros, Josselin Lupette, Juliette Jouhet. Extraction and Quantification of Lipids from Plant or Algae. Marechal Eric. Plastids: Methods and Protocols. Series: Methods in Molecular Biology, 1829, Springer, pp.213-240, 2018, Methods in Molecular Biology, 978-1-4939-8654-5; 978-1-4939-8653-8. ⟨10.1007/978-1-4939-8654-5_15⟩. ⟨hal-02190907⟩
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