A spliceosomal twin intron (stwintron) participates in both exon skipping and evolutionary exon loss - Archive ouverte HAL
Article Dans Une Revue Scientific Reports Année : 2019

A spliceosomal twin intron (stwintron) participates in both exon skipping and evolutionary exon loss

Napsugár Kavalecz
  • Fonction : Auteur
Norbert Ág
  • Fonction : Auteur
Levente Karaffa
  • Fonction : Auteur
Michel Flipphi
  • Fonction : Auteur
Erzsébet Fekete
  • Fonction : Auteur

Résumé

Spliceosomal twin introns (stwintrons) are introns where any of the three consensus sequences involved in splicing is interrupted by another intron (internal intron). In Aspergillus nidulans, a donor-disrupted stwintron (intron-1) is extant in the transcript encoding a reticulon-like protein. The orthologous transcript of Aspergillus niger can be alternatively spliced; the exon downstream the stwintron could be skipped by excising a sequence that comprises this stwintron, the neighbouring intron-2, and the exon bounded by these. This process involves the use of alternative 3' splice sites for the internal intron, the resulting alternative intervening sequence being a longer 3'-extended stwintron. In 29 species of Onygenales, a multi-step splicing process occurs in the orthologous transcript, in which a complex intervening sequence including the stwintron and neigbouring intron-2, generates by three splicing reactions a "second order intron" which must then be excised with a fourth splicing event. The gene model in two species can be envisaged as one canonical intron (intron-1) evolved from this complex intervening sequence of nested canonical introns found elsewhere in Onygenales. Postulated splicing intermediates were experimentally verified in one or more species. This work illustrates a role of stwintrons in both alternative splicing and the evolution of intron structure.

Dates et versions

hal-02183789 , version 1 (15-07-2019)

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Citer

Napsugár Kavalecz, Norbert Ág, Levente Karaffa, Claudio Scazzocchio, Michel Flipphi, et al.. A spliceosomal twin intron (stwintron) participates in both exon skipping and evolutionary exon loss. Scientific Reports, 2019, 9 (1), pp.9940. ⟨10.1038/s41598-019-46435-x⟩. ⟨hal-02183789⟩
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