Transfer RNA maturation in Chlamydomonas mitochondria, chloroplast and the nucleus by a single RNase P protein. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Plant Journal Année : 2016

Transfer RNA maturation in Chlamydomonas mitochondria, chloroplast and the nucleus by a single RNase P protein.

Résumé

The maturation of tRNA precursors involves the 5' cleavage of leader sequences by an essential endonuclease called RNase P. Beyond the ancestral ribonucleoprotein (RNP) RNase P, a second type of RNase P called PRORP (protein-only RNase P) evolved in eukaryotes. The current view on the distribution of RNase P in cells is that multiple RNPs, multiple PRORPs or a combination of both, perform specialised RNase P activities in the different compartments where gene expression occurs. Here, we identify a single gene encoding PRORP in the green alga Chlamydomonas reinhardtii while no RNP is found. We show that its product, CrPRORP, is triple-localised to mitochondria, the chloroplast and the nucleus. Its downregulation results in impaired tRNA biogenesis in both organelles and the nucleus. CrPRORP, as a single-subunit RNase P for an entire organism, makes up the most compact and versatile RNase P machinery described in either prokaryotes or eukaryotes.

Mots clés

Dates et versions

hal-02174133 , version 1 (04-07-2019)

Identifiants

Citer

Géraldine Bonnard, Anthony Gobert, Mathilde Arrivé, Franziska Pinker, Thalia Salinas, et al.. Transfer RNA maturation in Chlamydomonas mitochondria, chloroplast and the nucleus by a single RNase P protein.. Plant Journal, 2016, 87 (3), pp.270-280. ⟨10.1111/tpj.13198⟩. ⟨hal-02174133⟩
88 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More