Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo - Archive ouverte HAL
Article Dans Une Revue Methods in Molecular Biology Année : 2019

Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo

Résumé

In this chapter, we present a protocol to perform correlative light and electron microscopy (CLEM) on Caenorhabditis elegans embryos. We use a specific fixation method which preserves both the GFP fluorescence and the structural integrity of the samples. Thin sections are first analyzed by light microscopy to detect GFP-tagged proteins, then by transmission electron microscopy (TEM) to characterize the ultrastructural anatomy of cells. The superimposition of light and electron images allows to determine the subcellular localization of the fluorescent protein. We have used this method to characterize the roles of autophagy in the phagocytosis of apoptotic cells in C. elegans embryos. We analyzed in apoptotic cell and phagocytic cell the localization of the two homologs of LC3/GABARAP proteins, namely, LGG-1 and LGG-2.
Fichier non déposé

Dates et versions

hal-02173132 , version 1 (04-07-2019)

Identifiants

Citer

Céline Largeau, Renaud Legouis. Correlative Light and Electron Microscopy to Analyze LC3 Proteins in Caenorhabditis elegans Embryo. Methods in Molecular Biology, 2019, 1880, pp.281--293. ⟨10.1007/978-1-4939-8873-0_18⟩. ⟨hal-02173132⟩
16 Consultations
0 Téléchargements

Altmetric

Partager

More