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              <p>Objectives: Former industrial sites contaminated with Polycyclic Aromatic Hydrocarbons (PAHs) arean environmental and human health concern in Europe. These sites, often former gasworks and cokefactories, are close to or are nowadays part of urban areas and are persistent sources of pollution forthe underground water that have to be cleaned. PAHs are also a problem for the petroleum industry.The refining of crude oil, the transport, and the petrochemical industry can release PAHs in theenvironment, leading to health and environment risks.Bioremediation of PAHs in pore water and soils is possible and had already been done but is limitedby the low solubility of such molecules, which reduces their bioavailability. Surfactants can enhancethe apparent solubility of hydrophobic compounds, like PAHs, entrapping them by the formation ofmicelles. Chemically-produced surfactants, widely used in soil remediation (i.e. soil washingtechniques, etc.) are impaired by their low biocompatibility. Using biosurfactants, produced bybacteria, can improve this biocompatibility and then enhance PAHs bioavailability.The objective of this project is to treat an industrial soil and the groundwater sheet associated,impacted by PAHs with biosurfactant washing and biodegradation at the same time.The present work is part of the Bioxyval project that gathers multiple actors, from public and privatesectors, of the soil remediation area. The objective of the project is to develop and implementinnovative soil treatment methods, on a former industrial site, with multiple pollutions, includingPAHs (former coke factory).Innovative nature of the proposed topic: The aim of the project is to develop an innovativesolution for the remediation of the PAHs impacted soil by coupling a soil washing phase withbiosurfactants and a stimulated biodegradation phase of the PAHs solubilized in the water.Experimental approach: From the soil samples of the contaminated site, several bacterialcultures had been extracted and isolated. One of them shown abilities to produce biosurfactants andhad been selected for further experiments and the optimization of the biosurfactants production.For the optimization of the biosurfactant production, several series of analysis were made, to selectthe best carbon source, to find the most appropriate culture medium and nutrient balance and tosee the influence of others parameters like temperature or the presence of others bacteria isolates.For the PAHs degradation assays, tests were made with soil and water samples from thecontaminated site. Injections of the concentrated bacterial strain, of nutrients, or directlybiosurfactant were tested. The biosurfactant action was compared with some commercialsurfactants and biosurfactants.Results: The biosurfactant production tests show that the isolated bacterial culture was able toproduce biosurfactant from an insoluble or a soluble carbon source. The medium with glucose as thecarbon source was the one with the fastest production and was selected for further experiment.The presence of both nitrogen and phosphorus nutrient in the culture medium seems to bemandatory for the biosurfactant production for this bacterial isolate, an optimal balance betweennitrogen, phosphorus and glucose was found to optimize the speed of the reaction.The bacterial isolate seems able to produce biosurfactant in the presence of other bacteria.Biodegradation trials have already proved the high potential of bacteria for their ability to producebiosurfactant, which pushes the PAHs up to micellar state. On the water samples, evidences of theuse of the produced biosurfactant for the PAHs degradation had been found.The second phase of the project is to test both the biosurfactant production, the PAHs solubilizationand the PAHs biodegradation in soil column to see the influence of the physical parameters of thesoil on the remediation process. During the final step of this work, those lab trials will be extended toan in situ pilot treatment on an industrial site.</p>
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