Endocytosis Analysis by Flow Cytometry : Study of the Endocytosis of Fluoresceinylated Neoglycoproteins Via Membrane Lectins of Macrophages
Résumé
Twenty years ago, G. Ashwell, A. Morell and their coworkers showed that parenchymal cells of the liver are able to bind and to internalize glycoconjugates bearing galactosyl residues in a terminal non reducing position (Ashwell and Morell, 1982). Since, endogenous lectins have been characterized at the surface of a number of normal cells (hepatocytes, macrophages, monocytes, lymphocytes and endothelial cells) as well as tumor cells (Monsigny et al., 1983 and 1988).