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Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 1983

Protein-sugar interactions: Environmental effect on the fluorescence of O-(4-methylumbelliferyl)-glycosides

P. Midoux
F. Delmotte
  • Fonction : Auteur
J.Ph Grivet
  • Fonction : Auteur

Résumé

We have investigated the effect of 12 solvents and several amino acids on the fluorescence of O-(4-methylumbelliferyl)-glycosides. We showed that: i) the fluorescence quenching is not related to the dielectric constant of the solvents: the fluorescence intensity was maximal in water (d=80) and in acetic acid (d=6.2) and was at least ten times lower in acetone (d=21) and in dioxane (d=2.2); ii) the fluorescence of O-(4-methylumbelliferyl)-N-acetyl-β-glucosaminide is not quenched in the presence of various amino acids including arginine, asparagine, aspartate, histidine, leucine, phenylalanine and proline; iii) the fluorescence of O-(4-methylumbelliferyl)-glycoside is quenched by sulfur, phenol and indole amino acids or derivatives containing sulfur, phenol or indole groups. The changes in fluorescence intensities of O-(4-methylumbelliferyl)-glycosides upon binding to concanavalin A, wheat germ agglutinin and lysozyme are discussed with regard to the amino acid content of their binding sites.

Dates et versions

hal-02163727 , version 1 (24-06-2019)

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Citer

P. Midoux, F. Delmotte, J.Ph Grivet, M. Monsigny. Protein-sugar interactions: Environmental effect on the fluorescence of O-(4-methylumbelliferyl)-glycosides. Biochemical and Biophysical Research Communications, 1983, 110 (3), pp.926-933. ⟨10.1016/0006-291X(83)91051-3⟩. ⟨hal-02163727⟩
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