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Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 1984

The binding of monosaccharides to wheat germ agglutinin: Fluorescence and NMR investigations

P. Midoux
J.P Grivet
  • Fonction : Auteur
F. Delmotte
  • Fonction : Auteur

Résumé

The synthesis of N-acetyl- and N-trifluoroacetyl-glucosaminides was reported. The interaction of these compounds with wheat germ agglutinin, a plant lectin specific for N-acetyl-glucosamine and sialic acid, was investigated by two complementary approaches: 1H and 19F NMR, and fluorescence spectroscopy. This last technique relies on the existence of a competitive equilibrium involving the protein, the ligand and O-(methylumbelliferyl)-N-acetyl-glucosaminide, a fluorescent saccharide. The binding constants and the chemical shifts in the complex were determined and were related to the protein structure.

Dates et versions

hal-02163716 , version 1 (24-06-2019)

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P. Midoux, J.P Grivet, F. Delmotte, M. Monsigny. The binding of monosaccharides to wheat germ agglutinin: Fluorescence and NMR investigations. Biochemical and Biophysical Research Communications, 1984, 119 (2), pp.603-611. ⟨10.1016/s0006-291x(84)80292-2⟩. ⟨hal-02163716⟩
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