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Article Dans Une Revue Toxicology Letters Année : 2016

Effects of PVA-coated nanoparticles on human T helper cell activity

Résumé

Superparamagnetic iron oxide nanoparticles (SPION) are used as high-sensitive enhancer for magnetic resonance imaging, where they represent a promising tool for early diagnosis of destructive diseases such as rheumatoid arthritis (RA). Since we could demonstrate that professional phagocytes are activated by amino-polyvinyl-alcohol-coated-SPION (a-PVA-SPION), the study here focuses on the influence of a-PVA-SPION on human T cells activity. Therefore, primary human CD4+ T cells from RA patients and healthy subjects were treated with varying doses of a-PVA-SPION for 20h or 72h. T cells were then analyzed for apoptosis, cellular energy, expression of the activation marker CD25 and cell proliferation. Although, we observed that T cells from RA patients are more susceptible to low-dose a-PVA-SPION-induced apoptosis than T cells from healthy subjects, in both groups a-PVA-SPION do not activate CD4+ T cells per se and do not influence mitogen-mediated T cells activation with regard to CD25 expression and cell proliferation. Nevertheless, our results demonstrate that CD4+ T cells from RA patients and healthy subjects differ in their response to mitogen stimulation and oxygen availability. We conclude from our data, that a-PVA-SPION do neither activate nor significantly influence mitogen-stimulated CD4+ T cells activation and have negligible influence on T cells apoptosis.
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Dates et versions

hal-02163434 , version 1 (09-03-2021)

Identifiants

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Cindy Strehl, Saskia Schellmann, Lionel Maurizi, Margarethe Hofmann-Amtenbrink, Thomas Häupl, et al.. Effects of PVA-coated nanoparticles on human T helper cell activity. Toxicology Letters, 2016, 245, pp.52-58. ⟨10.1016/j.toxlet.2016.01.003⟩. ⟨hal-02163434⟩
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