Characterization of membrane lectins in human colon carcinoma cells by flow cytofluorometry, drug targeting and affinity chromatography. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Anticancer Research Année : 1987

Characterization of membrane lectins in human colon carcinoma cells by flow cytofluorometry, drug targeting and affinity chromatography.

Hj Gabius
  • Fonction : Auteur
R Engelhardt
  • Fonction : Auteur
T Hellmann
  • Fonction : Auteur
Patrick Midoux
G Nagel
  • Fonction : Auteur
K Vehmeyer
  • Fonction : Auteur

Résumé

Flow cytofluorometry and drug targeting with labelled neoglycoproteins are used as tools to probe for membrane lectins in two human adenocarcinoma cell lines. Both cell lines express activities for galactosides, glucosides and fucosides. Affinity chromatography on gels with immobilized sugar leads to purification of an alpha-galactoside-binding protein at an apparent molecular weight of 64 kDa that also binds to lactose, maltose and fucose and exhibits Ca2+-requirement for binding, a beta-galactoside-binding protein without Ca2+-requirement at an apparent molecular weight of 14 kDa, and an alpha-glucosyl-binding protein without Ca2+-requirement at an apparent molecular weight of 34 kDa from both cell lines. The description of membrane lectins, documented here for the first human tumor cell lines, is an initial step towards a lectin-based improvement of the clinical management of human colon adenocarcinoma.
Fichier non déposé

Dates et versions

hal-02163405 , version 1 (24-06-2019)

Identifiants

  • HAL Id : hal-02163405 , version 1
  • PUBMED : 3566177

Citer

Hj Gabius, R Engelhardt, T Hellmann, Patrick Midoux, M. Monsigny, et al.. Characterization of membrane lectins in human colon carcinoma cells by flow cytofluorometry, drug targeting and affinity chromatography.. Anticancer Research, 1987, 7 (1), pp.109-112. ⟨hal-02163405⟩
19 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More