Endocytosis of alpha 1-acid glycoprotein variants by human monocytic lineage cells. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biology of the Cell Année : 1993

Endocytosis of alpha 1-acid glycoprotein variants by human monocytic lineage cells.

V. Carpentier
  • Fonction : Auteur
Patrick Midoux

Résumé

Human alpha 1-acid glycoprotein (AGP or orosomucoid) is a major glycoprotein of plasma. AGP can be separated on immobilized concanavalin A into three variants bearing none (AGP A), one (AGP B) or two (AGP C) biantennary glycans. In this paper, we show, using flow cytometry and confocal microscopy, that AGP C which is eluted from concanavalin A with mannose, binds to human monocytes, monocyte-derived macrophages as well as human promonocytic cell lines such as THP1 or U937. Conversely HL60, a promyelocytic cell line, does not express the surface AGP C binding protein. AGP C is internalized and degraded with an efficiency depending on the state of differentiation of these cells. In contrast, AGP A which is not recognized by concanavalin A, does not bind to any of these cells.

Dates et versions

hal-02161453 , version 1 (20-06-2019)

Identifiants

Citer

V. Carpentier, Patrick Midoux, M. Monsigny, A.C. Roche. Endocytosis of alpha 1-acid glycoprotein variants by human monocytic lineage cells.. Biology of the Cell, 1993, 77 (2), pp.187-93. ⟨10.1016/S0248-4900(05)80187-X⟩. ⟨hal-02161453⟩
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