Molecular cloning of a novel widely expressed human 80 kDa 17 β -hydroxysteroid dehydrogenase IV - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 1995

Molecular cloning of a novel widely expressed human 80 kDa 17 β -hydroxysteroid dehydrogenase IV

J Adamski
  • Fonction : Auteur
Thierry Normand
F Leenders
  • Fonction : Auteur
D Monté
  • Fonction : Auteur
A. Bégué
  • Fonction : Auteur
D. Stehelin
  • Fonction : Auteur
P Jungblut
  • Fonction : Auteur
Y de Launoit
  • Fonction : Auteur

Résumé

Reactions of oestrogens and androgens at position C-17 are catalysed by 17 beta-hydroxysteroid dehydrogenases (17 beta-HSDs). Cloning of the cDNA of a novel human 17 beta-HSD IV and expression of its mRNA are described. A probe derived from the recently discovered porcine 17 beta-oestradiol dehydrogenase (17 beta-EDH) was used to isolate a 2.6 kb human cDNA encoding a continuous protein of 736 amino acids of high (84%) similarity to the porcine 17 beta-EDH. The calculated molecular mass of the human enzyme is 79,595 Da. Other sequence similarities shared by the two enzymes are: an N-terminal sequence which is similar to that of members of the short-chain alcohol dehydrogenase family; amino acids 343-607 which are similar to the C-terminal domains of a trifunctional Candida tropicalis enzyme and the FOX2 gene product of Saccharomyces cerevisiae; amino acids 596-736 which are similar to human sterol carrier protein 2. The previously cloned human 17 beta-HSD I, II and III are less than 25% identical with 17 beta-HSD IV. mRNA for HSD IV is a single species of 3.0 kb, present in many tissues with highest concentrations in liver, heart, prostate and testes. When over-expressed in mammalian cells, the human 17 beta-HSD IV enzyme displays a specific unidirectional oxidative 17 beta-HSD activity.

Dates et versions

hal-02160026 , version 1 (19-06-2019)

Identifiants

Citer

J Adamski, Thierry Normand, F Leenders, D Monté, A. Bégué, et al.. Molecular cloning of a novel widely expressed human 80 kDa 17 β -hydroxysteroid dehydrogenase IV. Biochemical Journal, 1995, 311 (2), pp.437-443. ⟨10.1042/bj3110437⟩. ⟨hal-02160026⟩

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