Format du dépôt |
Fichier |
Type de dépôt |
Article dans une revue |
Résumé |
en
Viral metagenomics and high throughput sequence mining have revealed unexpected diversity, and the potential presence, of parvoviruses in animals from all phyla. Among arthropods, this diversity highlights the poor knowledge that we have regarding the evolutionary history of densoviruses. The aim of this study was to explore densovirus diversity in a small arthropod pest belonging to Acari, the two-spotted spider mite Tetranychus urticae, while using viral metagenomics based on virus-enrichment. Here, we present the viromes obtained from T. urticae laboratory populations made of contigs that are attributed to nine new potential viral species, including the complete sequence of a novel densovirus. The genome of this densovirus has an ambisens genomic organization and an unusually compact size with particularly small non-structural proteins and a predicted major capsid protein that lacks the typical PLA2 motif that is common to all ambidensoviruses described so far. In addition, we showed that this new densovirus had a wide prevalence across populations of mite species tested and a genomic diversity that likely correlates with the host phylogeny. In particular, we observed a low densovirus genomic diversity between the laboratory and natural populations, which suggests that virus within-species evolution is probably slower than initially thought. Lastly, we showed that this novel densovirus can be inoculated to the host plant following feeding by infected mites, and circulate through the plant vascular system.
These findings offer new insights into densovirus prevalence, evolution, and ecology.
|
Titre |
en
A New Prevalent Densovirus Discovered in Acari. Insight from Metagenomics in Viral Communities Associated with Two-Spotted Mite (Tetranychus urticae) Populations
|
Sous-Titre |
en
Metagenomics in Viral Communities Associated with Two-Spotted Mite (Tetranychus urticae) Populations
|
Auteur(s)
|
Sarah François
1
, Doriane Mutuel
1
, Alison Duncan
2
, Leonor Rodrigues
3
, Celya Danzelle
2
, Sophie Lefevre
2
, Inês Santos
3
, Marie Frayssinet
1
, Emmanuel Fernandez
4
, Denis Filloux
4
, Philippe Roumagnac
4
, Rémy Froissart
5
, Mylène Ogliastro
1
1
DGIMI -
Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier]
( 189647 )
- Place Eugène Bataillon 34095 Montpellier cedex 5
- France
-
Institut National de la Recherche Agronomique UMR1333 ( 92114 )
;
-
Université Montpellier 2 - Sciences et Techniques ( 92690 )
;
-
Université de Montpellier ( 410122 )
2
UMR ISEM -
Institut des Sciences de l'Evolution de Montpellier
( 29770 )
- Place E. Bataillon CC 064 34095 Montpellier Cedex 05
- France
-
Centre de Coopération Internationale en Recherche Agronomique pour le Développement UMR116 ( 11574 )
;
-
École Pratique des Hautes Études ( 110691 )
;
-
Université Paris Sciences et Lettres ( 564132 )
;
-
Université de Montpellier ( 410122 )
;
-
Institut de recherche pour le développement [IRD] : UR226 ( 431575 )
;
-
Centre National de la Recherche Scientifique UMR5554 ( 441569 )
3
Centre for Ecology, Evolution and Environmental Changes (cE3c)
( 562190 )
- Portugal
-
Universidade de Lisboa = University of Lisbon = Université de Lisbonne ( 1114557 )
4
UMR BGPI -
Biologie et Génétique des Interactions Plante-Parasite
( 73810 )
- CIRAD
UMR-BGPI TA A-54/K - Campus International de Baillarguet - 34398 Montpellier Cedex 5
- France
-
Centre de Coopération Internationale en Recherche Agronomique pour le Développement UMR54 ( 11574 )
;
-
Institut National de la Recherche Agronomique UMR0385 ( 92114 )
;
-
Centre international d'études supérieures en sciences agronomiques ( 92699 )
;
-
Institut national d’études supérieures agronomiques de Montpellier ( 474617 )
5
MIVEGEC-GeneSys -
Du gène à l'écosystème
( 1052804 )
- France
-
Pathogènes, Environnement, Santé Humaine ( 1052802 )
;
-
Maladies infectieuses et vecteurs : écologie, génétique, évolution et contrôle ( 166573 )
;
-
Université de Montpellier UMR5290 ( 410122 )
;
-
Centre National de la Recherche Scientifique UMR5290 ( 441569 )
;
-
Institut de Recherche pour le Développement 224 ( 451860 )
|
Licence |
Paternité
|
Langue du document |
Anglais
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Non spécifiée
|
Date de publication |
2019
|
Volume |
11
|
Numéro |
3
|
Page/Identifiant |
233
|
Public visé |
Scientifique
|
Sous-type de document pour les Articles |
Research article
|
Domaine(s) |
-
Sciences de l'environnement/Biodiversité et Ecologie
-
Informatique [cs]/Bio-informatique [q-bio.QM]
-
Sciences du Vivant [q-bio]/Microbiologie et Parasitologie/Virologie
|
Financement |
-
Plant Health and Environment INRA division (SPE); H2020-EU VIROPLANT grant [773567]; PHC-PESSOA grant [38014YC]
|
Indexation contrôlée |
-
parvovirus
-
arthropode
-
densovirus
-
métagénomique
|
Mots-clés |
en
viral metagenomics, virus diversity, virus phylogeny, agricultural pests, arthropod, mite, viral communities, viral ecology, arthropods, parvoviruses, insect, metagenomics
fr
parvovirus, arthropode, densovirus, métagénomique
|
DOI |
10.3390/v11030233 |
ProdINRA |
464797 |
UT key WOS |
000464409200006 |