Article Dans Une Revue Biotechnology and Bioengineering Année : 2018

Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous ferredoxin-ferredoxin reductase systems

Résumé

To boost aldehyde deformylating oxygenase (ADO) activity in a Cupriavidus necator strain expressing a synthetic alkane pathway, the expression of two ferredoxin-ferredoxin reductase systems was tested. The genes of a native fd/FNR-like system were identified in C. necator and expressed in a previously engineered alka(e)ne producing strain. The improved production of alka(e)nes in this Re2061-pMAB1 strain confirmed the activity of the native Fd/FNR system in C. necator. Concomitantly, the expression of the heterologous system from Synechococcus elongatus was investigated identically, leading to a second strain, Re2061-pMAB2. In the bioreactor, the aldehyde production was strongly reduced compared with the original alka(e)ne producer, leading to alka(e)nes production up to 0.37 and 1.48 g/L (22 and 82 mg/g(CDW))(,) respectively. The alka(e)ne production yield of Re2061-pMAB2 accounted for 15% of the theoretical yield. We report here the highest level and yield of alka(e)nes production by an engineered bacterium to date.

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hal-02154335 , version 1 (01-04-2025)

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Lucie Crepin, Manon Barthe, Florence Leray, Stéphane Guillouet. Alka(e)ne synthesis in Cupriavidus necator boosted by the expression of endogenous and heterologous ferredoxin-ferredoxin reductase systems. Biotechnology and Bioengineering, 2018, 115 (10), pp.2576-2584. ⟨10.1002/bit.26805⟩. ⟨hal-02154335⟩
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