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Article Dans Une Revue Journal of Virology Année : 2012

HIV-1 Antisense Transcription Is Preferentially Activated in Primary Monocyte-Derived Cells

Résumé

In this study, an antisense luciferase-expressing human immunodeficiency virus type 1 (HIV-1) molecular clone was used to infect primary cells. We found that antisense transcription activity from the 3' long terminal repeat (LTR) was significantly more abundant in monocyte-derived cells than in activated T lymphocytes. Moreover, by analyzing antisense transcription in infected monocyte-derived dendritic cells (MDDCs), we observed that the majority of HIV-1-infected MDDCs with significant antisense transcription activity did not produce Gag. We also confirmed that the negative-strand-encoded antisense protein (ASP) was expressed in monocyte-derived cells.

Dates et versions

hal-02146251 , version 1 (03-06-2019)

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Sylvain Laverdure, Antoine Gross, Charlotte Arpin-André, Isabelle Clerc, Bruno Beaumelle, et al.. HIV-1 Antisense Transcription Is Preferentially Activated in Primary Monocyte-Derived Cells. Journal of Virology, 2012, 86 (24), pp.13785-13789. ⟨10.1128/JVI.01723-12⟩. ⟨hal-02146251⟩
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