Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cell Stem Cell Année : 2009

Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal

Rana Abou-Khalil
  • Fonction : Auteur
Fabien Le Grand
Giorgia Pallafacchina
  • Fonction : Auteur
Samuel Valable
François-Jérôme Authier
Michael A Rudnicki
  • Fonction : Auteur
Romain K Gherardi
  • Fonction : Auteur
Fabrice Chretien
  • Fonction : Auteur
Athanassia Sotiropoulos
  • Fonction : Auteur
Peggy Lafuste
Didier Montarras
  • Fonction : Auteur
Bénédicte Chazaud

Résumé

Mechanisms governing muscle satellite cell withdrawal from cell cycle to enter into quiescence remain poorly understood. We studied the role of angiopoietin 1 (Ang1) and its receptor Tie-2 in the regulation of myogenic precursor cell (mpc) fate. In human and mouse, Tie-2 was preferentially expressed by quiescent satellite cells in vivo and reserve cells (RCs) in vitro. Ang1/Tie-2 signaling, through ERK1/2 pathway, decreased mpc proliferation and differentiation, increased the number of cells in G0, increased expression of RC-associated markers (p130, Pax7, Myf-5, M-cadherin), and downregulated expression of differentiation-associated markers. Silencing Tie-2 had opposite effects. Cells located in the satellite cell neighborhood (smooth muscle cells, fibroblasts) upregulated RC-associated markers by secreting Ang1 in vitro. In vivo, Tie-2 blockade and Ang1 overexpression increased the number of cycling and quiescent satellite cells, respectively. We propose that Ang1/Tie-2 signaling regulates mpc self-renewal by controlling the return to quiescence of a subset of satellite cells.

Dates et versions

hal-02142762 , version 1 (28-05-2019)

Identifiants

Citer

Rana Abou-Khalil, Fabien Le Grand, Giorgia Pallafacchina, Samuel Valable, François-Jérôme Authier, et al.. Autocrine and paracrine angiopoietin 1/Tie-2 signaling promotes muscle satellite cell self-renewal. Cell Stem Cell, 2009, 5 (3), pp.298--309. ⟨10.1016/j.stem.2009.06.001⟩. ⟨hal-02142762⟩
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