Integrated pH Measurement during Reaction Monitoring with Dual-Reception 1 H– 31 P NMR Spectroscopy
Résumé
Simultaneous detection of 1H and 31P NMR signals through a dual detection scheme with two receivers allows monitoring both the signals of a molecule and the pH of the solution through the resonance of the inorganic phosphate. We evaluate here the method in terms of sensitivity and ease of implementation, and show that the additional information obtained without any loss of information or increase in measuring time can be of practical importance in a number of biochemical systems