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Article Dans Une Revue Retrovirology Année : 2008

Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging

Résumé

The human immunodeficiency virus type 1 (HIV-1) encodes several regulatory proteins, notably Vpr which influences the survival of the infected cells by causing a G2/M arrest and apoptosis. Such an important role of Vpr in HIV-1 disease progression has fuelled a large number of studies, from its 3D structure to the characterization of specific cellular partners. However, no direct imaging and quantification of Vpr-Vpr interaction in living cells has yet been reported. To address this issue, eGFP- and mCherry proteins were tagged by Vpr, expressed in HeLa cells and their interaction was studied by two photon fluorescence lifetime imaging microscopy and fluorescence correlation spectroscopy.

Dates et versions

hal-02122506 , version 1 (07-05-2019)

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Pascal Didier, Jean-Pierre Clamme, Emmanuel Schaub, Delphine Muriaux, Charlotte Cabanne, et al.. Direct Vpr-Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging. Retrovirology, 2008, 5 (1), pp.87. ⟨10.1186/1742-4690-5-87⟩. ⟨hal-02122506⟩
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