The mechanism for the ATP-induced uncoupling of respiration in mitochondria of the yeast Saccharomyces cerevisiae - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biochemical Journal Année : 1995

The mechanism for the ATP-induced uncoupling of respiration in mitochondria of the yeast Saccharomyces cerevisiae

Résumé

We have recently reported that ATP induces an uncoupling pathway in Saccharomyces cerevisiae mitochondria [Prieto, Bouillaud, Ricquier and Rial (1992) Eur. J. Biochem. 208, 487-491]. The presence of this pathway would explain the reported low efficiency of oxidative phosphorylation in S. cerevisiae, and may represent one of the postulated energy-dissipating mechanisms present in these yeasts. In this paper we demonstrate that ATP exerts its action in two steps: first, at low ATP/Pi ratios, it increases the respiratory-chain activity, probably by altering the kinetic properties of cytochrome c oxidase. Second, at higher ATP/Pi ratios, an increase in membrane permeability leads to a collapse in membrane potential. The ATP effect on cytochrome c oxidase corroborates a recent report showing that ATP interacts specifically with yeast cytochrome oxidase, stimulating its activity [Taanman and Capaldi (1993) J. Biol. Chem. 268, 18754-18761].

Domaines

Mycologie
Fichier principal
Vignette du fichier
The mechanism for the ATP-induced uncoupling of respiration biochemj 1995.pdf (603.78 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02119253 , version 1 (14-10-2022)

Identifiants

Citer

Susana Prieto, Frédéric Bouillaud, Eduardo Rial. The mechanism for the ATP-induced uncoupling of respiration in mitochondria of the yeast Saccharomyces cerevisiae. Biochemical Journal, 1995, 307 (3), pp.657-661. ⟨10.1042/bj3070657⟩. ⟨hal-02119253⟩
20 Consultations
8 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More