Optimization of cell culture conditions for the evaluation of the biological activities of the tetrapeptide N-Acetyl-Ser-Asp-Lys-Pro, a natural hemoregulatory factor. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Growth Factors Année : 1993

Optimization of cell culture conditions for the evaluation of the biological activities of the tetrapeptide N-Acetyl-Ser-Asp-Lys-Pro, a natural hemoregulatory factor.

Résumé

The present study attempts to define the difficulties in evaluating the properties of the hemoregulatory peptide AcSDKP using in vitro assays. In fact, in the presence of sera, which are generally added to basic culture media, AcSDKP is catabolized by proteases present in the serum. The kinetics of AcSDKP degradation depends on the nature and on the concentration of the added serum. In in vitro conditions, the half life of this peptide can be increased by the addition of 1 microM captopril, a metalloprotease inhibitor. Thus, these points need to be considered in designing experiments to study the effects of AcSDKP.
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Dates et versions

hal-02114636 , version 1 (29-04-2019)

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  • HAL Id : hal-02114636 , version 1
  • PUBMED : 8217216

Citer

C. Grillon, M Lenfant, J. Wdzieczak-Bakala. Optimization of cell culture conditions for the evaluation of the biological activities of the tetrapeptide N-Acetyl-Ser-Asp-Lys-Pro, a natural hemoregulatory factor.. Growth Factors, 1993, 9 (2), pp.133-8. ⟨hal-02114636⟩
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