MAP65-3 Microtubule-Associated Protein Is Essential for Nematode-Induced Giant Cell Ontogenesis in Arabidopsis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Plant cell Année : 2008

MAP65-3 Microtubule-Associated Protein Is Essential for Nematode-Induced Giant Cell Ontogenesis in Arabidopsis

Philippe Lecomte
Fabien Jammes
  • Fonction : Auteur
S. Pagnotta
Emilie Andrio
  • Fonction : Auteur
Janice de Almeida Engler
  • Fonction : Auteur
  • PersonId : 1206613
Nicolas Marfaing
  • Fonction : Auteur
Pierre Abad
  • Fonction : Auteur

Résumé

The infection of plants by obligate parasitic nematodes constitutes an interesting model for investigating plant cytoskeleton functions. Root knot nematodes have evolved the ability to manipulate host functions to their own advantage by redifferentiating root cells into multinucleate and hypertrophied feeding cells. These giant cells result from repeated rounds of karyokinesis without cell division. Detailed functional analyses demonstrated that Arabidopsis thaliana Microtubule-Associated Protein65-3 (MAP65-3) was essential for giant cell ontogenesis and that cytokinesis was initiated but not completed in giant cells. In developing giant cells, MAP65-3 was associated with a novel kind of cell plate-the giant cell mini cell plate-that separates daughter nuclei. In the absence of functional MAP65-3, giant cells developed but failed to fully differentiate and were eventually destroyed. These defects in giant cells impaired the maturation of nematode larvae. Thus, MAP65-3 is essential for giant cell development during root knot nematode infection. Subcellular localization of MAP65-3 and analysis of microtubule organization in the dyc283 T-DNA map65-3 mutant demonstrated that MAP65-3 played a critical role in organizing the mitotic microtubule array during both early and late mitosis in all plant organs. Here, we propose a model for the role of MAP65-3 in giant cell ontogenesis.

Dates et versions

hal-02111994 , version 1 (26-04-2019)

Identifiants

Citer

Marie-Cécile Caillaud, Philippe Lecomte, Fabien Jammes, Michael Quentin, S. Pagnotta, et al.. MAP65-3 Microtubule-Associated Protein Is Essential for Nematode-Induced Giant Cell Ontogenesis in Arabidopsis. The Plant cell, 2008, 20 (2), pp.423-437. ⟨10.1105/tpc.107.057422⟩. ⟨hal-02111994⟩
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