Sucrose 6F-phosphate phosphorylase: a novel insight in the human gut microbiome - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Microbial Genomics Année : 2019

Sucrose 6F-phosphate phosphorylase: a novel insight in the human gut microbiome

Résumé

The human gut microbiome plays an essential role in maintaining human health including in degradation of dietary fibres and carbohydrates further used as nutrients by both the host and the gut bacteria. Previously, we identified a polysaccharide utilization loci (PUL) involved in sucrose and raffinose family oligosaccharide (RFO) metabolism from one of the most common Firmicutes present in individuals, Ruminococcus gnavus E1. One of the enzymes encoded by this PUL was annotated as a putative sucrose phosphate phosphorylase (RgSPP). In the present study, we have in-depth characterized the heterologously expressed RgSPP as sucrose 6F-phosphate phosphorylase (SPP), expanding our knowledge of the glycoside hydrolase GH13_18 subfamily. Specifically, the enzymatic characterization showed a selective activity on sucrose 6F-phosphate (S6FP) acting both in phosphorolysis releasing alpha-d-glucose-1-phosphate (G1P) and alpha-d-fructose-6-phosphate (F6P), and in reverse phosphorolysis from G1P and F6P to S6FP. Interestingly, such a SPP activity had never been observed in gut bacteria before. In addition, a phylogenetic and synteny analysis showed a clustering and a strictly conserved PUL organization specific to gut bacteria. However, a wide prevalence and abundance study with a human metagenomic library showed a correlation between SPP activity and the geographical origin of the individuals and, thus, most likely linked to diet. Rgspp gene overexpression has been observed in mice fed with a high-fat diet suggesting, as observed for humans, that intestine lipid and carbohydrate microbial metabolisms are intertwined. Finally, based on the genomic environment analysis, in vitro and in vivo studies, results provide new insights into the gut microbiota catabolism of sucrose, RFOs and S6FP.
Fichier principal
Vignette du fichier
tauzin-microbial-genomics-2019.pdf (2.92 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-02095894 , version 1 (10-04-2019)

Licence

Paternité

Identifiants

Citer

Alexandra Tauzin, Laetitia Bruel, Elisabeth Laville, Cendrine Nicoletti, David Navarro, et al.. Sucrose 6F-phosphate phosphorylase: a novel insight in the human gut microbiome. Microbial Genomics, 2019, ⟨10.1099/mgen.0.000253⟩. ⟨hal-02095894⟩
116 Consultations
116 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More