Dynamic spatial ability is supposed to be involved in a critical process of air traffic controllers, namely conflict detection. The present paper aims at testing whether dynamic spatial ability improves with air traffic control training and/or experience. We designed a laboratory task to assess the performance in predicting if two moving disks would collide or not. We conducted a cross-sectional study with four groups of participants : ATCO trainees at the beginning (N=129), middle (N=80) or end of training (N=66) and experienced ATCOs (N=14). Results suggested on one hand that air traffic control training leads to a decrease in the number of extremely high proportions of undetected collisions from the middle of the training. On the other hand, air traffic control operational experience leads to a decrease in the number of extremely high proportions of falsely detected collisions.