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Journal Articles Nature Communications Year : 2019

Automating multimodal microscopy with NanoJ-Fluidics

Pedro Almada
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Pedro M. Pereira
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Siân Culley
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Christina L Dix
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Buzz Baum
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Romain F Laine
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Ricardo Henriques
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Combining and multiplexing microscopy approaches is crucial to understand cellular events, but requires elaborate workflows. Here, we present a robust, open-source approach for treating, labelling and imaging live or fixed cells in automated sequences. NanoJ-Fluidics is based on low-cost Lego hardware controlled by ImageJ-based software, making high-content, multimodal imaging easy to implement on any microscope with high reproducibility. We demonstrate its capacity on event-driven, super-resolved live-to-fixed and multiplexed STORM/DNA-PAINT experiments.


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Dates and versions

hal-02073341 , version 1 (19-03-2019)



Pedro Almada, Pedro M. Pereira, Siân Culley, Ghislaine Caillol, Fanny Boroni-Rueda, et al.. Automating multimodal microscopy with NanoJ-Fluidics. Nature Communications, 2019, 10 (1), pp.1223. ⟨10.1038/s41467-019-09231-9⟩. ⟨hal-02073341⟩
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