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Article Dans Une Revue Planta Année : 2019

Diversity and evolution of cytochromes P450 in stramenopiles

Résumé

Comparative genomic analysis of cytochromes P450 revealed high diversification and dynamic changes in stramenopiles, associated with transcriptional responsiveness to various environment stimuli. Abstract Comparative genomic and molecular evolution approaches were used to characterize P450 diversity in stramenopiles. Phylogenetic analysis further supported the high diversity of P450 in stramenopiles. Three clans were defined, that is, CYP51 clan and CYP97 clan present in brown algae, diatoms and Nannochloropsis gaditana, CYP5014 clan present in oomycetes. Two ancient families, CYP51 and CYP97 showed a close relationship with red algae, green algae and higher plants. Gene gain and loss patterns revealed that six CYP families-CYP51, CYP97, CYP5160, CYP5021, CYP5022, and CYP5165-predated the split of brown algae and diatoms. After they diverged, diatoms gained more CYP families, especially in the cold-adapted species Fragilariopsis cylindrus, in which eight new CYP families were found. Selection analysis revealed that the expanded CYP51 family in the brown alga Cladosiphon okamuranus exhibited a more relaxed selection constraint compared with those of other brown algae and diatoms. Our RNA-seq data further evidenced that most of P450s in Saccharina japonica are highly expressed in large sporophytes, which could potentially promote the large kelp formation in this life stage. Analysis of Ectocarpus siliculosus and diatom microarray transcriptomes showed that many P450s are responsive to stress, limited nutrient or light quality, suggesting their pivotal roles in detoxification or metabolic processes under adverse environmental stimuli. The information provided in this study will be helpful in designing functional experiments and interpreting P450 roles in this particular lineage.
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Dates et versions

hal-02065007 , version 1 (01-07-2019)

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Linhong Teng, Xiao Fan, David R. Nelson, Wentao Han, Xiao Zhang, et al.. Diversity and evolution of cytochromes P450 in stramenopiles. Planta, 2019, 249 (3), pp.647-661. ⟨10.1007/s00425-018-3028-1⟩. ⟨hal-02065007⟩
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