Involvement of Nitric Oxide Synthase in antiproliferative activity of macrophages: Induction of the enzyme requires two different kinds of signal acting synergistically - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue ~ Pergamon Int. J. lmmunopharmac Année : 1994

Involvement of Nitric Oxide Synthase in antiproliferative activity of macrophages: Induction of the enzyme requires two different kinds of signal acting synergistically

Résumé

Activated rodent macrophages inhibit microorganism and tumour cell growth through a high output of nitric oxide; generated by an isoform of nitric oxide synthase which is induced, for example, in murine macrophages, by concomitant stimulation with interferon-y (IFN-y) and lipopolysaccharide (LPS). We show here that LPS could be replaced as a co-stimulant by the mycobacterial derivative muramyl dipeptide (MDP) in macrophages, and by interleukin-I (IL-1) in EMT-6 adenocarcinoma cells. Moreover, our results indicate that nitric oxide synthase RNA synthesis required either simultaneous or sequential exposure to IFN-y and MDP/IL-1; whereas exposure to MDP/IL-1 followed by exposure to IFN-y was ineffective. Thus, two kinds of signal could be distinguished: IFN-y on the one hand, acting first in an irreversible way, and LPS, MDP, IL-1 on the other hand, which seemed to be permanently required for continuous transcription of the nitric oxide synthase gene.
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hal-02050463 , version 1 (27-02-2019)

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  • HAL Id : hal-02050463 , version 1

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Benoît Chénais, Jean-Pierre Tenu. Involvement of Nitric Oxide Synthase in antiproliferative activity of macrophages: Induction of the enzyme requires two different kinds of signal acting synergistically. ~ Pergamon Int. J. lmmunopharmac, 1994, 16. ⟨hal-02050463⟩
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