Closed-Form Expression of the Fourier Ring-Correlation for Single-Molecule Localization Microscopy - Archive ouverte HAL
Communication Dans Un Congrès Année : 2019

Closed-Form Expression of the Fourier Ring-Correlation for Single-Molecule Localization Microscopy

Thanh-An Pham
  • Fonction : Auteur
  • PersonId : 1026958
Daniel Sage
Michael Unser

Résumé

Single-molecule localization microscopy (SMLM) is a popular microscopic technique that achieves super resolution imaging by localizing individual blinking molecules in thousands of frames. Therefore , the reconstructed high-resolution image is a combination of millions of point sources. This particular computational reconstruction leads to the question of the estimation of the image resolution. Fourier-ring correlation (FRC) is the standard tool for assessing the resolution. It has been proposed for SMLM by computing a discrete correlation in the Fourier domain. In this work, we derive a closed-form expression to compute the continuous FRC. Our implementation provides an exact FRC and an alternative to compute a parameter-free FRC. In addition, it gives insights on the discrepancy of the discrete FRC and yields a rule to select its parameters such as the spatial sampling step or the width of the kernel used as density estimator.
Fichier principal
Vignette du fichier
CLOSED-FORM EXPRESSION OF THE FOURIER RING-CORRELATION FOR SINGLE-MOLECULE LOCALIZATION MICROSCOPY.pdf (330.09 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-02048083 , version 1 (25-02-2019)

Identifiants

  • HAL Id : hal-02048083 , version 1

Citer

Thanh-An Pham, Emmanuel Soubies, Daniel Sage, Michael Unser. Closed-Form Expression of the Fourier Ring-Correlation for Single-Molecule Localization Microscopy. ISBI 2019 - IEEE International Symposium on Biomedical Imaging, Apr 2019, Venise, Italy. ⟨hal-02048083⟩
857 Consultations
610 Téléchargements

Partager

More