Article Dans Une Revue Chemical Engineering Transactions Année : 2018

Bio-Layer interferometry method for quantification of Rubisco in plant extracts

Résumé

Biolayer interferometry (BLI) with biosensors has been tested for measuring the concentration of a protein in different media. Interferometry is based on the interference model of the light reflected by a surface. When the surface of an optical fibre is modified by a bounded compound, interference model changes as a function of the increase of the path length. This technique of protein quantification represents an alternative to the classical methods such as electrophoresis, spectrophotometry, immune-enzymatic tests (ELISA) or HPLC. The main advantages of this method are its specificity, its sensitivity and the promptness of the analysis. In this work, a measuring method for Ribulose-1,5-bisphosphate Carboxylase Oxydase (Rubisco) from different media has been developed. The most important conclusion is that Rubisco can be efficiently determined by this technique. Moreover, experimental results have shown the strong influence of pH on the measurements.

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Dates et versions

hal-02016731 , version 1 (12-02-2019)

Identifiants

  • HAL Id : hal-02016731 , version 1
  • PRODINRA : 459645

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Maria Usta Bony, Maria Aurora Fernandez, Stéphane Mathe. Bio-Layer interferometry method for quantification of Rubisco in plant extracts. Chemical Engineering Transactions, 2018, 65, 6 p. ⟨hal-02016731⟩
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