Characterization of two fungal lipoxygenases expressed in Aspergillus oryzae. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Bioscience and Bioengineering Année : 2018

Characterization of two fungal lipoxygenases expressed in Aspergillus oryzae.

Shinobu Takagi
  • Fonction : Auteur correspondant

Résumé

Two fungal lipoxygenase genes were cloned from a rice pathogen, Magnaporthe salvinii, and the take-all fungus, Gaeumannomyces graminis var. tritici, and successfully expressed in Aspergillus oryzae in secreted form. The lipoxygenases expressed, termed MLOX and GLOX, were purified and characterized to evaluate suitability for industrial applications. Both enzymes were active broadly at pH 4-11 and had optimum temperatures around 60 °C, but they were largely different in substrate specificity. Where MLOX was active broadly on arachidonic acid, EPA and DHA, and even on derivatives of fatty acids, such as methyl linoleate or linoleoyl alcohol, GLOX was more specific to linoleic acid and linolenic acid. The most remarkable difference between the two fungal LOXs was the positional and stereo-specificity of oxygenation reactions on polyunsaturated fatty acids. When using linoleic acid as the substrate, the product of MLOX was 9S-hydroperoxy-(E,Z)-octadecadienoic acid (9S(E,Z)-HPODE), on the other hand, the product of GLOX was 13R(E,Z)-HPODE. The enzymes were evaluated for a couple of potential applications and found to be effective on bleaching colored compounds such as carotenoids.
Fichier non déposé

Dates et versions

hal-02010136 , version 1 (06-02-2019)

Identifiants

Citer

Akiko Sugio, Kenji Matsui, Shinobu Takagi. Characterization of two fungal lipoxygenases expressed in Aspergillus oryzae.. Journal of Bioscience and Bioengineering, 2018, 126 (4), pp.436-444. ⟨10.1016/j.jbiosc.2018.04.005⟩. ⟨hal-02010136⟩
14 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More