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Article Dans Une Revue eLife Année : 2018

CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors

Manuela d'Alessandro
Magali Richard
Christian Stigloher
Vincent Gache
Thomas Boulin
Jean-Louis Bessereau
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Résumé

The assembly of neurotransmitter receptors in the endoplasmic reticulum limits the number of receptors delivered to the plasma membrane, ultimately controlling neurotransmitter sensitivity and synaptic transfer function. In a forward genetic screen conducted in the nematode C. elegans, we identified crld-1 as a gene required for the synaptic expression of ionotropic acetylcholine receptors (AChR). We demonstrated that the CRLD-1A isoform is a membrane-associated ER-resident protein disulfide isomerase (PDI). It physically interacts with AChRs and promotes the assembly of AChR subunits in the ER. Mutations of Creld1, the human ortholog of crld-1a, are responsible for developmental cardiac defects. We showed that Creld1 knockdown in mouse muscle cells decreased surface expression of AChRs and that expression of mouse Creld1 in C. elegans rescued crld-1a mutant phenotypes. Altogether these results identify a novel and evolutionarily-conserved maturational enhancer of AChR biogenesis, which controls the abundance of functional receptors at the cell surface.
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Dates et versions

hal-02009626 , version 1 (16-11-2021)

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Manuela d'Alessandro, Magali Richard, Christian Stigloher, Vincent Gache, Thomas Boulin, et al.. CRELD1 is an evolutionarily-conserved maturational enhancer of ionotropic acetylcholine receptors. eLife, 2018, 7, ⟨10.7554/eLife.39649⟩. ⟨hal-02009626⟩
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