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Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2018

Precise tracking of vaccine-responding T-cell clones reveals convergent and personalized response in identical twins

Mikhail V Pogorelyy
  • Fonction : Auteur
Anastasia A Minervina
  • Fonction : Auteur
Anastasiia L Sycheva
  • Fonction : Auteur
Ekaterina A Komech
  • Fonction : Auteur
Elena I Kovalenko
  • Fonction : Auteur
Galina G Karganova
  • Fonction : Auteur
Evgeniy S Egorov
  • Fonction : Auteur
Alexander Yu Komkov
  • Fonction : Auteur
Dmitriy M Chudakov
  • Fonction : Auteur
Ilgar Z Mamedov
  • Fonction : Auteur
Thierry Mora
Yuri B Lebedev
  • Fonction : Auteur

Résumé

T-cell receptor (TCR) repertoire data contain information about infections that could be used in disease diagnostics and vaccine development, but extracting that information remains a major challenge. Here we developed a statistical framework to detect TCR clone proliferation and contraction from longitudinal repertoire data. We applied this framework to data from three pairs of identical twins immunized with the yellow fever vaccine. We identified 500-1500 responding TCRs in each donor and validated them using three independent assays. While the responding TCRs were mostly private, albeit with higher overlap between twins, they could be well predicted using a classifier based on sequence similarity. Our method can also be applied to samples obtained post-infection, making it suitable for systematic discovery of new infection-specific TCRs in the clinic.
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hal-01974035 , version 1 (08-01-2019)

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Mikhail V Pogorelyy, Anastasia A Minervina, Maximilian Puelma Touzel, Anastasiia L Sycheva, Ekaterina A Komech, et al.. Precise tracking of vaccine-responding T-cell clones reveals convergent and personalized response in identical twins. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (50), pp.12704-12709. ⟨10.1073/pnas.1809642115⟩. ⟨hal-01974035⟩
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