Precise tracking of vaccine-responding T-cell clones reveals convergent and personalized response in identical twins - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 2018

Precise tracking of vaccine-responding T-cell clones reveals convergent and personalized response in identical twins

Mikhail V Pogorelyy
  • Function : Author
Anastasia A Minervina
  • Function : Author
Anastasiia L Sycheva
  • Function : Author
Ekaterina A Komech
  • Function : Author
Elena I Kovalenko
  • Function : Author
Galina G Karganova
  • Function : Author
Evgeniy S Egorov
  • Function : Author
Alexander Yu Komkov
  • Function : Author
Dmitriy M Chudakov
  • Function : Author
Ilgar Z Mamedov
  • Function : Author
Thierry Mora
  • Function : Author
  • PersonId : 847757
Yuri B Lebedev
  • Function : Author

Abstract

T-cell receptor (TCR) repertoire data contain information about infections that could be used in disease diagnostics and vaccine development, but extracting that information remains a major challenge. Here we developed a statistical framework to detect TCR clone proliferation and contraction from longitudinal repertoire data. We applied this framework to data from three pairs of identical twins immunized with the yellow fever vaccine. We identified 500-1500 responding TCRs in each donor and validated them using three independent assays. While the responding TCRs were mostly private, albeit with higher overlap between twins, they could be well predicted using a classifier based on sequence similarity. Our method can also be applied to samples obtained post-infection, making it suitable for systematic discovery of new infection-specific TCRs in the clinic.
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Dates and versions

hal-01974035 , version 1 (08-01-2019)

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Attribution - CC BY 4.0

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Mikhail V Pogorelyy, Anastasia A Minervina, Maximilian Puelma Touzel, Anastasiia L Sycheva, Ekaterina A Komech, et al.. Precise tracking of vaccine-responding T-cell clones reveals convergent and personalized response in identical twins. Proceedings of the National Academy of Sciences of the United States of America, 2018, 115 (50), pp.12704-12709. ⟨10.1073/pnas.1809642115⟩. ⟨hal-01974035⟩
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