Purification of the Main Ginsenosides from a French Crop of Panax Quinquefolium L. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Liquid Chromatography and Related Technologies Année : 2006

Purification of the Main Ginsenosides from a French Crop of Panax Quinquefolium L.

Résumé

Active components of Panax quinquefolium L. roots (French growing) have been separated by centrifugal partition chromatography (CPC). Five pure ginsenosides, namely Rb1, Re, Rd, Gypenoside XVII, and Fl 1, have been isolated in one step, using a biphasic mixture of ethyl acetate / 1-butanol / water and gradient elution. Part of the effluent was monitored with an evaporative light scattering detector (ELSD), for direct control of the collected fractions, allowing an easy detection of these molecules with low or no absorbance in the UV. Isolated ginsenosides have been identified by comparison with standards and by H and 13C NMR studies.
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Dates et versions

hal-01968159 , version 1 (02-01-2019)

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L. Le Men-Olivier, Jean-Hugues Renault, P. Thepenier, J. Jacquier, M. Zeches-Hanrot, et al.. Purification of the Main Ginsenosides from a French Crop of Panax Quinquefolium L.. Journal of Liquid Chromatography and Related Technologies, 2006, 18 (8), pp.1655-1662. ⟨10.1080/10826079508009302⟩. ⟨hal-01968159⟩

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