Oligomycins A and E, major bioactive secondary metabolites produced by Streptomyces sp. strain HG29 isolated from a Saharan soil - Archive ouverte HAL Access content directly
Journal Articles Journal of Medical Mycology = Journal de Mycologie Médicale Year : 2018

Oligomycins A and E, major bioactive secondary metabolites produced by Streptomyces sp. strain HG29 isolated from a Saharan soil

Abstract

An actinobacterial strain, HG29, with potent activity against pathogenic, toxigenic and phytopathogenic fungi was isolated from a Saharan soil sample of Algeria. On the basis of morphological and chemotaxonomic characteristics, the strain was classified in the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.3% with Streptomyces gancidicus NBRC 15412T. The comparison of its cultural and physiological characteristics with this species revealed significant differences. Moreover, the phylogenetic tree showed that strain HG29 forms a distinct phyletic line within the genus Streptomyces. Production of antifungal activity was investigated by following kinetics in shake broth. The highest antifungal activity was obtained after five days of fermentation, and in the dichloromethane extract. Two active compounds, NK1 and NK2, were purified by HPLC using a C18 column. Their chemical structures were identified through nuclear magnetic resonance experiments and mass spectrometry as oligomycins E and A, respectively, which have not been reported to be produced by S. gancidicus. The two bioactive compounds exhibited significant antifungal activity in vitro, showing minimal inhibitory concentrations (MICs) values between 2 and 75μg/mL.
Fichier principal
Vignette du fichier
Khebizi_21138.pdf (908.93 Ko) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-01963621 , version 1 (15-01-2019)

Identifiers

Cite

N. Khebizi, H. Boudjella, Christian Bijani, N. Bouras, H. P. Klenk, et al.. Oligomycins A and E, major bioactive secondary metabolites produced by Streptomyces sp. strain HG29 isolated from a Saharan soil. Journal of Medical Mycology = Journal de Mycologie Médicale, 2018, 28 (1), pp.150-160. ⟨10.1016/j.mycmed.2017.10.007⟩. ⟨hal-01963621⟩
66 View
229 Download

Altmetric

Share

Gmail Facebook X LinkedIn More