Cold Atmospheric Plasma Parameters Investigation for Drug Delivery Applications
Résumé
Cold atmospheric plasmas (CAPs) have demonstrated to be quite useful tools in biomedical
applications and their relevance in medicine is getting more and more recognized. Drug delivery
in cancer cells combining low cytotoxicity and high efficiency remains to be optimized with
physical techniques. Several studies have shown that plasma could be used to deliver
chemotherapy [1] or nucleic acids [2] via enhancement of cell permeabilization. We investigated
the parameters needed for helium plasma to induce cell membrane permeabilization on cancer
cells for drug delivery purposes. 10,000 plasma pulses were used at a frequency of 100 Hz for
100 s with an applied voltage of 14 kV. Both plasma electric field and endocytosis involvement
in molecular uptake were also studied [3]. Kinetics of plasma-induced permeabilization were
studied. Highest permeabilization efficiency was observed when propidium iodide was added
after treatment (up to 40% of propidium iodide positive cells in both HeLa and 4T1). No
significant toxicity was observed in these conditions as the viability in HeLa cells was measured
to be of 80%. Doxorubicin was used as an anticancer drug. A treatment combining doxorubicin
and plasma decreased the concentration of doxorubicin needed to achieve 50% of cytotoxicity by
enhancing doxorubicin uptake in cancer cells.
References
[1] W. Zhu, S.-J. Lee, N. J. Castro, D. Yan, M. Keidar, and L. G. Zhang, Scientific Report, 6,
(2016).
[2] M. Jinno, Y. Ikeda, H. Motomura, Y. Kido and S. Satoh. Archives Biochemistry
Biophysics, 605, 59-66 (2016)
[3] V. Vijayarangan, A. Delalande, S. Dozias, J.-M. Pouvesle, C. Pichon, and E.