Kinetic modeling of light limitation and sulfur deprivation effects in the induction of hydrogen production with Chlamydomonas reinhardtii : Part I. Model development and parameter identification - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biotechnology and Bioengineering Année : 2009

Kinetic modeling of light limitation and sulfur deprivation effects in the induction of hydrogen production with Chlamydomonas reinhardtii : Part I. Model development and parameter identification

Swanny Fouchard
  • Fonction : Auteur
  • PersonId : 1277767
  • IdRef : 10465886X
Jeremy Pruvost
Benoit Degrenne
  • Fonction : Auteur
Mariana Titica
Jack Legrand

Résumé

Chlamydomonas reinhardtii is a green micro-alga capable of turning its metabolism towards H 2 production under specific conditions. However this H 2 production, narrowly linked to the photosynthetic process, results from complex metabolic reactions highly dependent on the environmental conditions of the cells. A kinetic model has been developed to relate culture evolution from standard photo-synthetic growth to H 2 producing cells. It represents transition in sulfur-deprived conditions, known to lead to H 2 production in Chlamydomonas reinhardtii, and the two main processes then induced which are an over-accumulation of intracellular starch and a progressive reduction of PSII activity for anoxia achievement. Because these phenomena are directly linked to the photosynthetic growth, two kinetic models were associated, the first (one) introducing light dependency (Haldane type model associated to a radiative light transfer model), the second (one) making growth a function of available sulfur amount under extra-cellular and intracellular forms (Droop formulation). The model parameters identification was realized from experimental data obtained with especially designed experiments and a sensitivity analysis of the model to its parameters was also conducted. Model behavior was finally studied showing interdependency between light transfer conditions, photo-synthetic growth, sulfate uptake, photosynthetic activity and O 2 release, during transition from oxygenic growth to anoxic H 2 production conditions.
Fichier principal
Vignette du fichier
2009_Bio&Bio_SdepriPartI_Fouchard_HAL.pdf (330.26 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01940666 , version 1 (24-04-2020)

Identifiants

Citer

Swanny Fouchard, Jeremy Pruvost, Benoit Degrenne, Mariana Titica, Jack Legrand. Kinetic modeling of light limitation and sulfur deprivation effects in the induction of hydrogen production with Chlamydomonas reinhardtii : Part I. Model development and parameter identification. Biotechnology and Bioengineering, 2009, 102 (1), pp.232-245. ⟨10.1002/bit.22034⟩. ⟨hal-01940666⟩
55 Consultations
167 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More