Journal Articles Scientific Reports Year : 2018

Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis

Abstract

Acyl-CoA:diacylglycerol acyltransferases 3 (DGAT3) are described as plant cytosolic enzymes synthesizing triacylglycerol. Their protein sequences exhibit a thioredoxin-like ferredoxin domain typical of a class of ferredoxins harboring a [2Fe-2S] cluster. The Arabidopsis thaliana DGAT3 (AtDGAT3; At1g48300) protein is detected in germinating seeds. The recombinant purified protein produced from Escherichia coli, although very unstable, exhibits DGAT activity in vitro. A shorter protein version devoid of its N-terminal putative chloroplast transit peptide, Δ46AtDGAT3, was more stable in vitro, allowing biochemical and spectroscopic characterization. The results obtained demonstrate the presence of a [2Fe-2S] cluster in the protein. To date, AtDGAT3 is the first metalloprotein described as a DGAT.

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hal-01938601 , version 1 (28-11-2018)

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Laure Aymé, Simon Arragain, Michel Canonge, Sebastien S. Baud, Nadia Touati, et al.. Arabidopsis thaliana DGAT3 is a [2Fe-2S] protein involved in TAG biosynthesis. Scientific Reports, 2018, 8 (1), pp.17254. ⟨10.1038/s41598-018-35545-7⟩. ⟨hal-01938601⟩
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