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Article Dans Une Revue Methods in Molecular Biology Année : 2018

Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System.

Résumé

Identification of membrane protein interactomes is a key issue to better understand how these molecules carry out their functions. However, protein-protein interactions using conventional interaction assays are particularly challenging for integral membrane proteins, because of their hydrophobic nature. Here we describe the membrane yeast two-hybrid (MbY2H) system, a powerful tool for identifying the interactors of membrane and membrane-associated proteins.
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hal-01927331 , version 1 (19-11-2018)

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Maxime Voisin, Emmanuel Vanrobays, Christophe Tatout. Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System.. Methods in Molecular Biology, 2018, 1840, pp.221-235. ⟨10.1007/978-1-4939-8691-0_16⟩. ⟨hal-01927331⟩
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