A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea. - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Plant cell Année : 1997

A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea.

Résumé

The self-incompatibility (SI) response in Brassica involves recognition of self-pollen by the papillar cells of the stigma and is mediated by the products of genes localized at the S (self-incompatibility) locus. Two S locus genes, SRK and SLG, are thought to encode components of a receptor complex present in the female partner. The putative gene product of SLA, a third S locus-linked gene that is expressed specifically in anthers, is a candidate for the male component of the SI recognition system. The identification of a mutant SLA allele, interrupted by a large insert resembling a ret-rotransposon, in self-compatible Brassica napos initially suggested that SLA played an essential role in the SI response. In this study, we have characterized an SLA allele from a self-compatible B. oleracea var acephala line and show that it too is interrupted by a large insert. However, analysis of seven B. oleracea var bofryfis lines exhibiting both self-compatible and self-incompatible phenotypes showed that these lines carry an S allele very similar or identical t o that of the B. oleracea var acephala line and that the SLA gene is interrupted by an insert in all seven lines. The insertion of the putative retrotransposon was shown t o interfere with gene expression, with no SLA transcripts being detected by RNA gel blot analysis in a self-incompatible B. oleracea var bofryfis line carrying an interrupted SLA gene. These data indicate that a functional SLA gene is not required for the SI response in Brassica.
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Dates et versions

hal-01926573 , version 1 (19-11-2018)

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Martine Pastuglia, Véronique Ruffio-Châble, Valérie Delorme, Thierry Gaude, Christian Dumas, et al.. A functional S locus anther gene is not required for the self-incompatibility response in Brassica oleracea.. The Plant cell, 1997, 9 (11), pp.2065-2076. ⟨10.1105/tpc.9.11.2065⟩. ⟨hal-01926573⟩
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