Postmortem change in muscle fibre autofluorescence
Résumé
Merisiers, F-91120 Gif sur Yvette, France
*Thierry.Astruc@clermont.inra.fr
Abstract – After slaughter, the muscle cells
undergo biochemical and physicochemical
changes which may affect their autofluorescence
characteristics. The postmortem metabolism
kinetic of rat EDL and soleus muscles was
assessed by glycogen depletion determination
while autofluorescent response of different
muscle fiber types was investigated by Deep UV
synchrotron microspectroscopy at slaughter and
and 24 hours postmortem. Following a 275 nm
excitation, emission fluorescence spectra showed
discrimination depending on postmortem time
(T0 versus T24h) on both muscles at 346 and
302 nm and to a lesser extent at 408 and 325 nm.
Taken individually, all fiber types are
discriminated but with variable accuracy, the
type IIA showing better separation of T0
comparing to T24h than other fiber types.
These results highlight the relevance of
using the autofluorescent response of muscle
cells to rapidly assess their state of postmortem
changes.