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Journal Articles Nature Communications Year : 2018

Megahertz data collection from protein microcrystals at an X-ray free-electron laser

Richard Bean
  • Function : Author
Marco Cammarata
Steffen Hauf
  • Function : Author
Yoonhee Kim
  • Function : Author
Romain Letrun
  • Function : Author
Grant Mills
  • Function : Author
Marco Ramilli
  • Function : Author
  • PersonId : 1007461
Tokushi Sato
  • Function : Author
Martin Weik
  • Function : Author
  • PersonId : 978699
Wajid Ehsan
  • Function : Author
Moritz Emons
  • Function : Author
Sergey Esenov
  • Function : Author
Hans Fangohr
  • Function : Author
Max Lederer
  • Function : Author
Luis Maia
  • Function : Author
Guido Palmer
  • Function : Author
Natascha Raab
  • Function : Author
Janusz Szuba
  • Function : Author
Jun Zhu
  • Function : Author
Claudiu Stan
  • Function : Correspondent author

Abstract

X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10-120 Hz, placing significant demands on beam time and sample consumption. We describe serial femtosecond crystallography experiments performed at the European XFEL, the first MHz repetition rate XFEL, delivering 1.128 MHz X-ray pulse trains at 10 Hz. Given the short spacing between pulses, damage caused by shock waves launched by one XFEL pulse on sample probed by subsequent pulses is a concern. To investigate this issue, we collected data from lysozyme microcrystals, exposed to a ~15 μm XFEL beam. Under these conditions, data quality is independent of whether the first or subsequent pulses of the train were used for data collection. We also analyzed a mixture of microcrystals of jack bean proteins, from which the structure of native, magnesium-containing concanavalin A was determined.
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Dates and versions

hal-01912078 , version 1 (17-07-2019)

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Luis Grünbein, Johan Bielecki, Alexander Gorel, Miriam Stricker, Richard Bean, et al.. Megahertz data collection from protein microcrystals at an X-ray free-electron laser. Nature Communications, 2018, 9 (1), pp.3487. ⟨10.1038/s41467-018-05953-4⟩. ⟨hal-01912078⟩
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