Article Dans Une Revue Journal of Clinical Microbiology Année : 2016

A Simple and Safe Protocol for Preparing Brucella Samples for Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis

Résumé

We describe a simple protocol to inactivate the biosafety level 3 (BSL3) pathogens Brucella prior to their analysis by matrix-assisted laser desorption ionization-time of flight mass spectrometry. This method is also effective for several other bacterial pathogens and allows storage, and eventually shipping, of inactivated samples; therefore, it might be routinely applied to unidentified bacteria, for the safety of laboratory workers.

Dates et versions

hal-01909349 , version 1 (31-10-2018)

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Jennifer Mesureur, Sébastien Ranaldi, Valérie Monnin, Victoria Girard, Sandrine Arend, et al.. A Simple and Safe Protocol for Preparing Brucella Samples for Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Analysis. Journal of Clinical Microbiology, 2016, 54 (2), pp.449 - 452. ⟨10.1128/JCM.02730-15⟩. ⟨hal-01909349⟩
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